کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2146242 1548322 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional interaction between hMYH and hTRADD in the TNF-α-mediated survival and death pathways of HeLa cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Functional interaction between hMYH and hTRADD in the TNF-α-mediated survival and death pathways of HeLa cells
چکیده انگلیسی


• We determine the interaction between hMYH and hTRADD.
• We examine changes in the level of hMYH–hTRADD interaction under TNF-α treatment.
• hTRADD–hMYH association is involved in the nuclear translocation of NFκB.
• hTRADD–hMYH complex influences the TNFR1–TRADD association.

The tumor necrosis factor (TNF) signaling pathway is a classical immune system pathway that plays a key role in regulating cell survival and apoptosis. The TNF receptor-associated death domain (TRADD) protein is recruited to the death domain of TNF receptor 1 (TNFR1), where it interacts with TNF receptor-associated factor 2 (TRAF2) and receptor-interacting protein (RIP) for the induction of apoptosis, necrosis, nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and mitogen-activated protein (MAP) kinase activation. In this study, we found that the human MutY homolog (hMYH) interacted with human TRADD (hTRADD) via the C-terminal domain of hMYH. Moreover, under conditions promoting TNF-α-induced cell death or survival in HeLa cells, this interaction was weakened or enhanced, respectively. The interaction between hMYH and hTRADD was important for signaling pathways mediated by TNF-α. Our results also suggested that the hTRADD–hMYH association was involved in the nuclear translocation of NFκB and formation of the TNFR1–TRADD complex. Thus, this study identified a novel mechanism through which the hMYH–hTRADD interaction may affect the TNF-α signaling pathway.ImplicationsIn HeLa cells, the hTRADD–hMYH interaction functioned in both cell survival and apoptosis pathways following TNF-α stimulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 777, July 2015, Pages 11–19
نویسندگان
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