کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2147208 | 1548402 | 2008 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNA sequence context affects UV-induced mutagenesis in Escherichia coli DNA sequence context affects UV-induced mutagenesis in Escherichia coli](/preview/png/2147208.png)
We investigated the effect of altering the DNA sequence surrounding a mutable target site on the production of ultraviolet light (UV) induced mutations. Site-directed base substitutions were incorporated on both sides of a TAA sequence encoding a UAA nonsense defect in the tyrA14 allele of Escherichia coli. This allele is readily revertable by UV and a total of eight different base substitution mutations can be recovered. Five different strains harboring DNA sequences allowing the formation of 5′-TT, 5′-CT and 5′-TA* photoproducts were constructed and exposed to UV. DNA sequence analysis was used to determine the spectrum of the revertants that were recovered. The results showed that changes at the 3′-base of a TT site were predominately T to C transitions and T to A transversions. However, unlike the TT site, a 5′-CT site produced a relatively high frequency of T to G transversions. In addition, T to A transversions that could not have been targeted by a cyclobutane-type or [6-4]-type pyrimidine dimer were produced; this result suggested that these mutations may be targeted by a TA* photoproduct. Also, a distinct strand bias was noted for two mechanistically identical base substitutions in a strain having a palindromic target sequence; this result may reflect an unequal damage distribution or processing of photoproducts as a consequence of asymmetric DNA replication. Finally, our results show that DNA sequences expected to allow the greatest density of UV-induced DNA damage produce the highest mutation frequencies. Overall, these findings provide new insights regarding the role of DNA photoproducts in UV mutagenesis.
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 638, Issues 1–2, 1 February 2008, Pages 154–161