کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2147245 1548403 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III
چکیده انگلیسی

It has been hypothesized that DNA mismatch repair (MMR) is coupled with DNA replication; however, the involvement of DNA polymerase III subunits in bacterial DNA MMR has not been clearly elucidated. In an effort to better understand the relationship between these 2 systems, the potential interactions between the Escherichia coli MMR protein and the clamp loader subunits of E. coli DNA polymerase III were analyzed by far western blotting and then confirmed and characterized by surface plasmon resonance (SPR) imaging. The results showed that the MMR key protein MutL could directly interact with both the individual subunits δ, δ′, and γ and the complex of these subunits (clamp loader). Kinetic parameters revealed that the interactions are strong and stable, suggesting that MutL might be involved in the recruitment of the clamp loader during the resynthesis step in MMR. The interactions between MutL, the δ and γ subunits, and the clamp loader were observed to be modulated by ATP. Deletion analysis demonstrated that both the N-terminal residues (1–293) and C-terminal residues (556–613) of MutL are required for interacting with the subunits δ and δ′. Based on these findings and the available information, the network of interactions between the MMR components and the DNA polymerase III subunits was established; this network provides strong evidence to support the notion that DNA replication and MMR are highly associated with each other.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 637, Issues 1–2, 1 January 2008, Pages 101–110
نویسندگان
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