کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2147585 | 1548419 | 2006 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Origins of human mitochondrial point mutations as DNA polymerase γ-mediated errors Origins of human mitochondrial point mutations as DNA polymerase γ-mediated errors](/preview/png/2147585.png)
Mitochondrial mutational spectra in human cells, tissues and derived tumors for bp 10,030–10,130 are essentially identical, suggesting a predominant mutagenic role for endogenous processes. We hypothesized that errors mediated by mitochondrial DNA polymerase γ were the primary sources of mutations. Point mutations created in this sequence by human DNA pol γ in vitro were thus compared to the eighteen mutational hotspots, all single base substitutions, previously found in human tissues. The set of concordant hotspots accounted for 83% of these in vivo mutational events. About half of these mutations are insensitive to prolonged heating of DNA during PCR and half increase proportionally with heating time at 98 °C. Primary misincorporation errors and miscopying errors past thermal denaturing products such as deaminated cytosines (uracils) thus appear to be of approximately equal importance. For the sequence studied, these data support the conclusion that, endogenous error mediated by DNA pol γ constitutes the primary source of mitochondrial point mutations in human tissues.
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 599, Issues 1–2, 25 July 2006, Pages 11–20