کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2147685 | 1548421 | 2006 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Novel mechanism for the radiation-induced bystander effect: Nitric oxide and ethylene determine the response in sponge cells Novel mechanism for the radiation-induced bystander effect: Nitric oxide and ethylene determine the response in sponge cells](/preview/png/2147685.png)
Until now the bystander effect had only been described in vertebrates. In the present study the existence of this effect has been demonstrated for the phylogenetically oldest metazoan phylum, the Porifera. We used the demosponge Suberites domuncula for the experiments in the two-chamber-system. The lower dish contained irradiated “donor” cells (single cells) and the upper dish the primmorphs (“recipient” primmorphs). The “donor” cells were treated with UV-B light (40 mJ/cm2) and 100 μM hydrogen peroxide (H2O2), factors that exist also in the natural marine aquatic environment of sponges; these factors caused a high level of DNA strand breaks followed by a reduced viability of the cells. If these cells were added to the “recipient” primmorphs these 3D-cell cultures started to undergo apoptosis. This effect could be abolished by the NO-specific scavenger PTIO and ethylene. The conclusion that NO is synthesized by the UV-B/H2O2-treated cells was supported analytically. The cDNA encoding the enzyme dimethylarginine dimethylaminohydrolase (DDAH) was isolated from the “donor” cells. High levels of DDAH transcripts were measured in UV-B/H2O2-treated “donor” cells while after ethylene treatment the steady-state level of expression drops drastically. We conclude that in the absence of ethylene the concentration of the physiological inhibitor for the NO synthase ADMA is low, due to the high level of DDAH. In consequence, high amounts of NO are released from “donor” cells which cause apoptosis in “recipient” primmorphs. In contrast, ethylene reduces the DDAH expression with the consequence of higher levels of ADMA which prevent the formation of larger amounts of NO. This study describes the radiation-induced bystander effect also for the most basal metazoans and demonstrates that this effect is controlled by the two gasses NO and ethylene.
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 597, Issues 1–2, 11 May 2006, Pages 62–72