Is there a relationship between early genotoxicity and life-history traits in Vicia faba exposed to cadmium-spiked soils?

The Vicia faba-micronucleus test is usually performed to assess the genotoxic potential of pure substances, effluents or water extracts from soil. It is also a relevant, early biological tool to detect mutagenic substances in crude soils. Nevertheless, the physiological meaning of such DNA damage for the plant in the long term remains to be elucidated. To know more about this, two experiments were carried out with the plant model V. faba. In a preliminary short-term experiment, seeds were exposed for five days to a soil spiked with different concentrations of CdCl2 in order to identify the concentration inducing the highest number of micronuclei without affecting plant growth. Thereafter, a long-term experiment was performed in the soil spiked with such a concentration (i.e. 510 μmol CdCl2 per kg dry soil), in which V. faba seeds were directly sowed and allowed to grow during 151 days. As a result, Cd-spiked soil did not affect seed-emergence time nor the growth-rate of the plants for the first two months. The first signs of toxicity appeared after the 70th day of exposure. Interestingly, exposed plants produced their first flower earlier and had a longer flowering period than did control ones. Nevertheless, total flower production was less abundant in exposed plants than in control plants. Moreover, fruits appeared in control plants whereas no fruit was formed in exposed plants. At last, exposed plants showed a reduced life time.Our results suggest that the micronucleus assay can provide a predictive biomarker of long-term deleterious effects in plants.