کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2148226 1089545 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recommended protocol for the BALB/c 3T3 cell transformation assay
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Recommended protocol for the BALB/c 3T3 cell transformation assay
چکیده انگلیسی

The present protocol has been developed for the BALB/c 3T3 cell transformation assay (CTA), following the prevalidation study coordinated by the European Centre for the Validation of Alternative Methods (ECVAM) and reported in this issue (Tanaka et al. [16]). Based upon the experience gained from this effort and as suggested by the Validation Management Team (VMT), some acceptance and assessment criteria have been refined compared to those used during the prevalidation study. The present protocol thus describes cell culture maintenance, the dose-range finding (DRF) experiment and the transformation assay, including cytotoxicity and morphological transformation evaluation. Use of this protocol and of the associated photo catalogue included in this issue (Sasaki et al. [17]) is recommended for the future conduct of the BALB/c 3T3 CTA.


► The detailed protocol of BALB/c 3T3 cell transformation assay in low serum medium.
► The medium in the early stage is MEM + FBS 10% and in the late stage is DMEM/F12 + insulin 2 μg/mL + FBS 2%.
► The prevalidation study demonstrated that an improved protocol has been developed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 744, Issue 1, 11 April 2012, Pages 30–35
نویسندگان
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