کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2149189 | 1089604 | 2007 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Genotoxic effects in M5 cells and Chinese hamster V79 cells after exposure to 7Li-beam (LET = 60 keV/μm) and correlation of their survival dynamics to nuclear damages and cell death Genotoxic effects in M5 cells and Chinese hamster V79 cells after exposure to 7Li-beam (LET = 60 keV/μm) and correlation of their survival dynamics to nuclear damages and cell death](/preview/png/2149189.png)
Chinese hamster V79 cell and a cell strain M5, derived from V79 cells and reported to be relatively resistant to γ-ray, hydrogen peroxide, and N-methyl-N-nitro-N-nitrosoguanidine (MNNG; a potent human carcinogen), were exposed to high LET 7Li-beam (LET = 60 keV/μm) at ∼90% confluent state in the dose range of 0–1 Gy. Effects of 7Li-beam exposure on cell survival, micronuclei induction (MN), chromosomal aberrations (CA) and apoptosis were compared in both the cell lines. A dose-dependent decline in survival for both the cell lines was noted, relatively less in M5 cells (mostly p < 0.01) indicating greater radio-resistance in this strain. The MN, CA and apoptosis increased in a dose-dependent manner in both V79 and M5 cells. Significant differences in various other parameters between these two cell lines were also noted. The relative intensity of DNA ladder, which is a useful marker for the determination of the extent of apoptosis induction, was much higher in V79 cells. A good correlation between the reduction of the surviving fractions and the increase in frequencies of MN or CA or apoptosis was noted for both the cell lines.
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 628, Issue 1, 30 March 2007, Pages 56–66