کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2153382 1090177 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Systematic screening of imaging biomarkers for the Islets of Langerhans, among clinically available positron emission tomography tracers
ترجمه فارسی عنوان
غربالگری سیستماتیک بیومارکرهای تصویربرداری برای جزایر لانگرهانس، از جمله توموگرافی انتشاراتی پوزیترون قابل دسترس
کلمات کلیدی
تصویربرداری سلول بتا، تصویربرداری جزیره، توموگرافی گسیل پوزیترون، پانکراس، جزایر لانگرهانس، غدد درون ریز
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی

IntroductionFunctional imaging could be utilized for visualizing pancreatic islets of Langerhans. Therefore, we present a stepwise algorithm for screening of clinically available positron emission tomography (PET) tracers for their use in imaging of the neuroendocrine pancreas in the context of diabetes.MethodsA stepwise procedure was developed for screening potential islet imaging agents. Suitable PET-tracer candidates were identified by their molecular mechanism of targeting. Clinical abdominal examinations were retrospectively analyzed for pancreatic uptake and retention. The target protein localization in the pancreas was assessed in silico by –omics approaches and the in vitro by binding assays to human pancreatic tissue.ResultsSix putative candidates were identified and screened by using the stepwise procedure. Among the tested PET tracers, only [11C]5-Hydroxy-tryptophan passed all steps. The remaining identified candidates were falsified as candidates and discarded following in silico and in vitro screening.ConclusionsOf the six clinically available PET tracers identified, [11C]5-HTP was found to be a promising candidate for beta cell imaging, based on intensity of in vivo pancreatic uptake in humans, and islet specificity as assessed on human pancreatic cell preparations. The flow scheme described herein constitutes a methodology for evaluating putative islet imaging biomarkers among clinically available PET tracers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Medicine and Biology - Volume 42, Issue 10, October 2015, Pages 762–769
نویسندگان
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