کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2153442 1090184 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of 99mTc-TRODAT-1 with high labeling yield in boiling water bath: A new formulation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Preparation of 99mTc-TRODAT-1 with high labeling yield in boiling water bath: A new formulation
چکیده انگلیسی

A new formulation for preparation of 99mTc-labeled tropane derivative, 99mTc-TRODAT-1, which is useful as a potential CNS dopamine transporter imaging agent, was evaluated and characterized.Preparation of 99mTc-TRODAT-1 was attained previously by a formulation in which vial has to be autoclaved at 121 °C for 30 min. It is highly desirable to further improve the preparation method by developing a simplified one vial formulation which will be labeled in boiling water bath (95 °C) for 15 min and a high labeling yield will be achieved. A formulation contained 10 μg of TRODAT-1, 20 μg tricine, 40 μg SnCl2 and 20 mg manitol was prepared. Labeling was performed at 95 °C for 15 min and radiochemical analysis involved ITLC and HPLC methods. The stability of radioconjugate was checked in the presence of human serum at 37 °C up to 24 h.99mTc-TRODAT-1 was prepared with a radiochemical purity of more than 95% and specific activity of 64.3 MBq/nmol. Biodistribution studies of this new formulation in rats revealed similar regional brain distribution as compared with those obtained with the previous preparation in which brain uptake was high in striatum and striatum to cerebellum ratio was high. Requiring no autoclave facility for labeling, this new formulation will significantly improve the using feasibility of this radiopharmaceutical in clinic.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Medicine and Biology - Volume 41, Issue 4, April 2014, Pages 317–321
نویسندگان
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