کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2153870 | 1090208 | 2012 | 6 صفحه PDF | دانلود رایگان |

Background and AimmiRNA is an important factor for tumorigenesis which could act as a potential molecular target for tumor diagnosis. The goal of this study was to explore a new method for visualizing the expression of let-7 in lung adenocarcinoma A549 cells by Cerenkov luminescence imaging (CLI) and gamma camera imaging.MethodsThe human sodium/iodine symporter (hNIS) and 3′-UTR sequence of the ras gene (RU) that complementarily binds to let-7 were cloned with hNIS serving as the reporter gene. The expression of hNIS regulated by let-7 in the fusion gene hNIS-RU was constructed; the let-7 primer (pri-let-7), which could specifically bind to RU and the mir-143 primer (pri-mir143) not binding with RU, was cloned. A549 cells were transfected with hNIS or hNIS-RU, and additional cells were cotransfected with hNIS-RU and different concentrations of pri-let-7 or pri-mir143. The cells were incubated with 740 kBq 131I-containing media for 1 h, 24 h after transfection. CLI, gamma camera imaging, and γ counting were subsequently conducted, and the correlation among CLI, gamma camera imaging, and γ counting was compared when cotransfected with pri-let-7.ResultsCLI, gamma camera imaging, and radioactive counting showed that hNIS-transfected A549 cells had significantly higher uptake of 131I compared to non-transfected cells. The uptake of 131I in hNIS-RU transfected A549 cells decreased to approximately 70% compared to hNIS-transfected cells, since hNIS-RU expression was suppressed by intracellular let-7. After cotransfection with hNIS-RU and various concentrations of pri-let-7, 131I uptake gradually decreased with increasing pri-let-7, while 131I uptake remained roughly unchanged in the presence of hNIS-RU cotransfected with different amounts of pri-mir143. CLI was highly correlated with gamma camera imaging (r2 = 0.9893) and radioactivity counting (0.9779).ConclusionsBased upon miRNA-regulated reporter genes which mediate the uptake of the radionuclide, both CLI and gamma camera imaging can noninvasively detect miRNA expression in cells, which may provide a new way for the visualization of miRNA expression.
Journal: Nuclear Medicine and Biology - Volume 39, Issue 7, October 2012, Pages 948–953