کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2159503 | 1090860 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: CD133 expression is not selective for tumor-initiating or radioresistant cell populations in the CRC cell line HCT-116 CD133 expression is not selective for tumor-initiating or radioresistant cell populations in the CRC cell line HCT-116](/preview/png/2159503.png)
Background and purposeCD133 is controversially discussed as putative (surrogate) marker for cancer stem/tumor-initiating cell populations (CSC/TIC) in epithelial tumors including colorectal carcinomas (CRCs). We studied CD133 expression in established CRC cell lines and examined in vitro behavior, radioresponse and in vivo tumor formation of CD133+/− subpopulations of one cell line of interest.Materials and methodsTen CRC cell lines were analyzed for CD133 expression using flow cytometry and Western blotting. CD133+ and CD133− HCT-116 subpopulations were separated by FACS and studied in 2-D and 3-D culture and colony formation assays after irradiation. Subcutaneous xenograft formation was monitored in NMRI (nu/nu) mice.Results and conclusionsCRC cell lines could be classified into three groups: (i) CD133−, (ii) CD133+ and (iii) those with two distinct CD133+ and CD133− subpopulations. Isolated CD133+/− HCT-116 subpopulations were studied relative to the original fraction. No difference was found in 2-D growth, spheroid formation or radioresponse in vitro. Also, tumor formation and growth rate did not differ for the sorted subpopulations. However, a subset of xenografts originated from CD133− HCT-116 showed a striking enrichment in the CD133+ fraction. Our data show that CD133 expression is not selective for sphere forming, tumor-initiating or radioresistant subpopulations in the HCT-116 CRC cell line. This implies that CD133 cannot be regarded as a CSC/TIC marker in all CRC cell lines and that functional measurements of tumor formation have to generally accompany CSC/TIC-directed mechanistic or therapeutic studies.
Journal: Radiotherapy and Oncology - Volume 94, Issue 3, March 2010, Pages 375–383