کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2163406 1091424 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intracranial Tumor Cell Migration and the Development of Multiple Brain Metastases in Malignant Melanoma 12
ترجمه فارسی عنوان
مهاجرت سلولهای تومور داخل سلولی و توسعه متاستازهای مغز چندگانه در ملانوم بدخیم 12
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی

INTRODUCTION: A majority of patients with melanoma brain metastases develop multiple lesions, and these patients show particularly poor prognosis. To develop improved treatment strategies, detailed insights into the biology of melanoma brain metastases, and particularly the development of multiple lesions, are needed. The purpose of this preclinical investigation was to study melanoma cell migration within the brain after cell injection into a well-defined intracerebral site. METHODS: A-07, D-12, R-18, and U-25 human melanoma cells transfected with green fluorescent protein were injected stereotactically into the right cerebral hemisphere of nude mice. Moribund mice were killed and autopsied, and the brain was evaluated by fluorescence imaging or histological examination. RESULTS: Intracerebral inoculation of melanoma cells produced multiple lesions involving all regions of the brain, suggesting that the cells were able to migrate over substantial distances within the brain. Multiple modes of transport were identified, and all transport modes were observed in all four melanoma lines. Thus, the melanoma cells were passively transported via the flow of cerebrospinal fluid in the meninges and ventricles, they migrated actively along leptomeningeal and brain parenchymal blood vessels, and they migrated actively along the surfaces separating different brain compartments. CONCLUSION: Migration of melanoma cells after initial arrest, extravasation, and growth at a single location within the brain may contribute significantly to the development of multiple melanoma brain metastases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Oncology - Volume 9, Issue 3, June 2016, Pages 211–218
نویسندگان
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