کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2165900 | 1091784 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential deregulation of astrocytic calcium signalling by amyloid-β, TNFα, IL-1β and LPS
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential deregulation of astrocytic calcium signalling by amyloid-β, TNFα, IL-1β and LPS Differential deregulation of astrocytic calcium signalling by amyloid-β, TNFα, IL-1β and LPS](/preview/png/2165900.png)
چکیده انگلیسی
In Alzheimer's disease (AD), astrocytes undergo complex morphological and functional changes that include early atrophy, reactive activation and Ca2+ deregulation. Recently, we proposed a mechanism by which nanomolar Aβ42 deregulates mGluR5 and InsP3 receptors, the key elements of astrocytic Ca2+ signalling toolkit. To evaluate the specificity of these changes, we have now investigated whether the effects of Aβ42 on Ca2+ signalling machinery can be reproduced by pro-inflammatory agents (TNFα, IL-1β, LPS). Here we report that Aβ42 (100 nM, 72 h) significantly increased mRNA levels of mGluR5, InsP3R1 and InsP3R2, whereas pro-inflammatory agents reduced expression of these specific mRNAs. Furthermore, DHPG-induced Ca2+ signals and store operated Ca2+ entry (SOCE) were augmented in Aβ42-treated cells due to up-regulation of a set of Ca2+ signalling-related genes including TRPC1 and TRPC4. Opposite changes were observed when astrocytes were treated with TNFα, IL-1β and LPS. Last, the effects observed on SOCE by treating wild-type astrocytes with Aβ42 were also identified in untreated astrocytes from 3ÃTg-AD animals, suggesting a link to the AD pathology. Our results demonstrate that effects of Aβ42 on astrocytic Ca2+ signalling differ from and may contrast to the effects of pro-inflammatory agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 55, Issue 4, April 2014, Pages 219-229
Journal: Cell Calcium - Volume 55, Issue 4, April 2014, Pages 219-229
نویسندگان
Virginia Ronco, Ambra A. Grolla, Toma N. Glasnov, Pier Luigi Canonico, Alexei Verkhratsky, Armando A. Genazzani, Dmitry Lim,