کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2166241 1091833 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A key role for dense granule secretion in potentiation of the Ca2+ signal arising from store-operated calcium entry in human platelets
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A key role for dense granule secretion in potentiation of the Ca2+ signal arising from store-operated calcium entry in human platelets
چکیده انگلیسی

Recent work has demonstrated a role for Na+/Ca2+ exchange in potentiation of the Ca2+ entry elicited through the human platelet store-operated channel by controlling a Mn2+-impermeable Ca2+ entry pathway. Here we demonstrate that this involves control over the secretion of dense granules by a Na+/Ca2+ exchanger (NCX) and so autocrine signalling between platelets. NCX inhibition reduced dense granule secretion. The reduction in SOCE elicited by NCX inhibition could be reversed by the addition of uninhibited donor cells, their releasate alone, or exogenous ADP and 5-HT. The use of specific receptor antagonists indicated that ATP, ADP and 5-HT all played a role in NCX-dependent autocrine signalling between platelets following thapsigargin stimulation, by activating Mn2+-impermeable Ca2+ entry pathways. These data provide further insight into the mechanisms underlying the known interrelationship between platelet Ca2+ signalling and dense granule secretion, and suggest an important role for the NCX in potentiation of platelet activation via dense granule secretion and so autocrine signalling. Our results caution the interpretation of platelet Ca2+ signalling studies involving pharmacological or other manipulations that do not assess possible effects on NCX activity and dense granule secretion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 45, Issue 5, May 2009, Pages 413–420
نویسندگان
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