کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2166646 1091874 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ICPBCZin: A red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
ICPBCZin: A red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions
چکیده انگلیسی

SummaryA new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3′,2′:3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 44, Issue 3, September 2008, Pages 270–275
نویسندگان
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