کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2166671 1091876 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-dimensional resolution of elementary Ca2+ signals by simultaneous multi-focal imaging
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Multi-dimensional resolution of elementary Ca2+ signals by simultaneous multi-focal imaging
چکیده انگلیسی

Elementary events such as puffs and sparks are cytosolic microdomains of Ca2+ from which cellular Ca2+ signals are constructed. Because of the tight localization and fast kinetics of elementary events, imaging studies have been hindered by instrumental limitations of confocal and deconvolution fluorescence microscopy which necessitate compromises between spatial and temporal resolution. Here, we describe a novel, yet simple ‘multi-focal’ fluorescence microscopy system that employs three high-speed cameras focused at different axial depths to enable 4-dimensional imaging with millisecond resolution. We demonstrate the utility of this system for studies of puffs in Xenopus oocytes by mapping the axial distribution of puff sites, by obtaining measurements of puff amplitudes undistorted by focus error, and by deriving deblurred images that reveal novel sub-micron jumps of Ca2+ release sites.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 43, Issue 4, April 2008, Pages 367–374
نویسندگان
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