کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2166843 | 1091895 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitochondria play a critical role in shaping the exocytotic response of rat pancreatic acinar cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mitochondria play a critical role in shaping the exocytotic response of rat pancreatic acinar cells Mitochondria play a critical role in shaping the exocytotic response of rat pancreatic acinar cells](/preview/png/2166843.png)
چکیده انگلیسی
We have previously demonstrated [M. Campos-Toimil, T. Bagrij, J.M. Edwardson, P. Thomas, Two modes of secretion in pancreatic acinar cells: involvement of phosphatidylinositol 3-kinase and regulation by capacitative Ca2+ entry, Curr. Biol. 12 (2002) 211-215] that in rat pancreatic acinar cells, Gd3+-sensitive Ca2+ entry is instrumental in governing which second messenger pathways control secretory activity. However, in those studies, we were unable to demonstrate a significant increase in cytoplasmic [Ca2+] during agonist application as a result of this entry pathway. In the present study, we combined pharmacology with ratiometric imaging of fura-2 fluorescence to resolve this issue. We found that 2 μM Gd3+ significantly inhibits store-mediated Ca2+ entry. Furthermore, both the protonophore, CCCP (5 μM) and the mitochondrial Ca2+-uptake blocker, RU360 (10 μM), led to an enhancement of the plateau phase of the biphasic Ca2+ response induced by acetylcholine (1 μM). This enhancement was completely abolished by Gd3+; and as has been previously shown for Gd3+, RU360 led to a switch to a wortmannin-sensitive form of exocytosis. Using MitoTracker Red staining we found a close association of mitochondria with the lateral plasma membrane. We propose that in rat pancreatic acinar cells, capacitative Ca2+ entry is targeted directly to mitochondria; and that as a result of Ca2+ uptake, these mitochondria release “third” messengers which both enhance exocytosis and suppress phosphatidylinositol 3-kinase-dependent secretion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 39, Issue 1, January 2006, Pages 57-63
Journal: Cell Calcium - Volume 39, Issue 1, January 2006, Pages 57-63
نویسندگان
Paul Thomas, Tanya Bagrij, Manuel Campos-Toimil, J. Michael Edwardson,