کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2166947 1092294 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A single bout of dynamic exercise by healthy adults enhances the generation of monocyte-derived-dendritic cells
ترجمه فارسی عنوان
یک تمرین تکاملی توسط بزرگسالان سالم باعث افزایش تولید سلول های دندریتیک مونوسیت می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• We compared mo-DCs generated from blood donated before and after brief exercise.
• Blood donated after exercise yielded nearly four times the number of mo-DCs.
• Mo-DCs generated after exercise could uptake antigen and stimulate autologous T-cells.
• Acute exercise may be a simple means to enhance mo-DC production for immunotherapy.

The ex vivo generation of monocyte-derived-dendritic cells (mo-DCs) has facilitated the use of DCs in immunotherapy research. However, low blood monocyte numbers frequently limit the manufacture of sufficient numbers of mo-DCs for subsequent experimental and clinical procedures. Because exercise mobilizes monocytes to the blood, we tested if acute dynamic exercise by healthy adults would augment the generation of mo-DCs without compromising their differentiation or function. We compared mo-DC generation from before- and after-exercise blood over 8-days of culture. Function was assessed by FITC-dextran uptake and the stimulation of autologous cytomegalovirus (pp65)-specific-T-cells. Supporting the hypothesis, we found a near fourfold increase in number of mo-DCs generated after-exercise. Furthermore, relative FITC-dextran uptake, differentiation rate, and stimulation of pp65-specific-T-cells did not differ between before- and after-exercise mo-DCs. We conclude that exercise enhances the ex vivo generation of mo-DCs without compromising their function, and so may overcome some limitations associated with manufacturing these cells for immunotherapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 295, Issue 1, May 2015, Pages 52–59
نویسندگان
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