کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2167025 | 1092302 | 2014 | 6 صفحه PDF | دانلود رایگان |
• Tregs and IL-17 producing T cells are both increased in CRC tissues.
• The ratio of the frequency of intratumoral Tregs/IL-17 producing T cells was decreased.
• Intratumoral Tregs suppress MMPs expression in CRC.
• Intratumoral Tregs may be associated with suppression of CRC metastasis after resection by overcoming IL-17 producing T cells.
With opposite immune activities, regulatory T cells (Tregs) and IL-17 producing T cells were accumulated in various malignant tumors and played critical roles in pathophysiologic course of these diseases. In this study, we investigated the mix-effect of the intratumoral Tregs and IL-17 producing T cells on metastasis of colorectal carcinoma (CRC) after resection. The frequency of intratumoral Tregs and IL-17A+ T cells, and the levels of FoxP3 and IL-17 mRNA were analyzed. The ratio of Tregs/IL-17A+ T cells and the ratio of FoxP3 mRNA/IL-17 mRNA were calculated. The activities of matrix metalloproteases (MMPs) in tumor tissues were analyzed. Meanwhile, Tregs from patient’s blood was co-cultured with human CRC cells in the presence of IL-17. MMPs protein and mRNA levels were determined after 48 or 24 h incubation. We found that Tregs and IL-17A+ T cells were accumulated in CRC. The ratio of Tregs/IL-17A+ T cells was decreased in CRC tissues. More intratumoral Tregs and less IL-17A+ T cells were associated with suppressed MMPs activities and decreased metastases score. In addition, vitro studies demonstrated that Tregs suppressed MMPs expression in the presence of IL-17. Our findings suggested the possibility that intratumoral Tregs protected against metastasis of CRC after resection through overcoming IL-17 producing T cells.
Journal: Cellular Immunology - Volume 287, Issue 2, February 2014, Pages 100–105