کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2167183 1549406 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Roles of Th17 cells in pulmonary granulomas induced by Schistosoma japonicum in C57BL/6 mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Roles of Th17 cells in pulmonary granulomas induced by Schistosoma japonicum in C57BL/6 mice
چکیده انگلیسی


• Schistosoma japonicum could induce IL-17 production in lungs.
• Th17 cells were the main cellular source of IL-17.
• IL-17 could be released by Th2 cells (IL-4 and IL-5 secreted CD4+ T cells).
• Blocking IL-17 could alleviate Schistosoma japonicum infection induced inflammation.

In schistosomiasis, limited information is available about the role of interleukin-17 (IL-17) in lung, despite the fact that this cytokine plays a crucial role during pro-inflammatory immune responses. In our study, we observed CD4+T cells changed after the infection. Furthermore, ELISA and FACS results revealed that Schistosomajaponicum infection could induce a large amount of IL-17 in mouse pulmonary lymphocytes. IL-17-producing cells, including Th17 cells, CD8+T (Tc) cells, γδT cells and natural killer T cells, was also associated with the development of lung inflammatory diseases. FACS results indicated that Th17 cell was the main source of IL-17 in the infected pulmonary lymphocytes after phorbol-12-myristate-13-acetate (PMA) and Ionomycin stimulation. Moreover, FACS results revealed that the percentage of Th17 cells continued to increase as over the course of S. japonicum infection. Additionally, cytokines co-expression results demonstrated that Th17 cells could express more IL-4 and IL-5 than IFN-γ. Reducing IL-17 activity by using anti-IL-17 ameliorated the damage and decreased infiltration of inflammatory cells in infected C57BL/6 mouse lungs. Collectively, these results suggest Th17 cells is the major IL-17-producing cells population and IL-17 contributes to pulmonary granulomatous inflammatory during the S. japonicum infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 285, Issues 1–2, September–October 2013, Pages 149–157
نویسندگان
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