کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2167195 | 1549410 | 2012 | 8 صفحه PDF | دانلود رایگان |

Yersinia pestis is the causative agent of plague. Cellular immunity seems to play an important role in defense against this disease. The subunit vaccine based on V (Lcr V) antigen has been proved to be immunogenic in animals and in humans. The multiple antigen peptide (MAP), incorporating all the relevant B and T cell epitopes is highly immunogenic in mice through intranasal route of immunization in PLGA particles containing CpG-ODN as an immunoadjuvant inducing humoral and mucosal immune response. In the present study, cell-mediated immune response using same MAP was studied in murine model. Primary and memory T cell responses were studied in outbred and inbred mice immunized intranasally with MAP in the presence of two immunoadjuvants (Murabutide and CpG-ODN). All the three compartments (Spleen, Lamina propria and Peyer’s patches) of the lymphoid system showed increased lymphoproliferative response. Highest lymphoproliferative response was observed especially with CpG-ODN. Cytokine profile in the culture supernatant showed highest Th1 and Th17 levels. FACS analysis showed expansion of both CD4+ and CD8+ T cells producing gamma-interferon, perforin and granzyme-B with major contribution from CD4+ T cells.
► F1 and V antigens are putative plague vaccine candidates.
► Subunit/peptide based vaccines can be made after identifying protective B and T cell epitopes of proteins.
► Multiple Antigen Peptide (MAP) was synthesized consisting of B and T cell epitopes of V antigen of Yersinia pestis.
► Cellular immune responses and cytokine profile were studied in vitro in murine model using intranasal immunization.
► FACS analysis proved expansion of CD4+ T cells and CD8+ T cells with IFN-γ, perforin and granzyme were studied.
Journal: Cellular Immunology - Volume 278, Issues 1–2, July–August 2012, Pages 55–62