کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2167249 1549414 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antigenic stimulation induces recombination activating gene 1 and terminal deoxynucleotidyl transferase expression in a murine T-cell hybridoma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Antigenic stimulation induces recombination activating gene 1 and terminal deoxynucleotidyl transferase expression in a murine T-cell hybridoma
چکیده انگلیسی

Secondary rearrangements of the T cell receptor (TCR) represent a genetic correction mechanism which changes T cell specificity by re-activating V(D)J recombination in peripheral T cells. Murine T-cell hybridoma A1.1 was employed to investigate whether antigenic stimulation induced re-expression of recombinase genes and altered TCR Vβ expression. Following repeated antigenic stimulation, A1.1 cells were induced to re-express recombination activating gene (RAG)1 and terminal deoxynucleotidyl transferase (TdT) which are generally considered prerequisite to TCR gene rearrangement. Accompanied with the significant changes in TCR mRNA levels over time, it is suggested that secondary rearrangements may be induced in A1.1 cells, which represent a mature T cell clone capable of re-expressing RAG genes and possesses the prerequisite for secondary V(D)J rearrangement.


► Murine T-cell hybridoma A1.1 displays an atypical secondary rearrangement of V–VDJ joint.
► Recombinase genes RAG and TdT can be induced to re-express in A1.1 cells following stimulated.
► A1.1 cells possesses the prerequisite for secondary V(D)J rearrangement.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 274, Issues 1–2, 2012, Pages 19–25
نویسندگان
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