کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2167456 1092333 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Increased sensitivity of cholera toxin B treated K562 cells to natural killer cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Increased sensitivity of cholera toxin B treated K562 cells to natural killer cells
چکیده انگلیسی
Cholera toxin B-subunit (CTB) treatment of K562 erythroleukemia cells increased their sensitivity to be killed by NK-92 cells with more than 10%, compared to untreated cells. A similar treatment of non-T, non-B acute lymphoblastic REH leukemia cells, known to be unsensitive to NK cell mediated cytotoxicity, did not have any impact at all. Visualization of the cross-linked gangliosideM1 (GM1) using fluorescent labeled CTB, indicated accumulation of the fluorescence to one cap and a few smaller patches in both type of cells. Additional cross-linking using anti-CTB antibodies further accentuated capping and increased lysis in the case of K562 cells. Blocking experiments performed with anti-MICA/B, ULBP-2 and/or CD59 antibodies could not inhibit the increased sensitivity mediated by CTB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 269, Issue 1, 2011, Pages 1-4
نویسندگان
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