کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2167588 1092342 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Killing effect of interleukin-13 receptor alpha 2 (IL-13Rα2) sensitized DC–CTL cells on human glioblastoma U251 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Killing effect of interleukin-13 receptor alpha 2 (IL-13Rα2) sensitized DC–CTL cells on human glioblastoma U251 cells
چکیده انگلیسی

Recent studies show that IL-13Rα2, a brain tumor-associated antigen for IL-13, may play a role in immunotherapy for glioblastoma. Thus, we stimulated the lymphocyte by monocyte-derived dendritic cells (DCs). The DCs were pulsed with IL-13Rα2 in vitro and then co-cultured with lymphocytes. After inducing cytotoxic T cells (CTLs) and co-culturing with U251 cells for 24 h in 96 wells, Cell Count Kit-8 (CCK-8) was added to every well equally. The optical density (OD) value was detected and recorded after 2 h. The DCs efficiently presented the antigen to the CTLs, resulting in CTLs activation and proliferation. The induced CTLs showed specific cytotoxic against U251 cells (P < 0.01). The results demonstrated that IL-13Rα2 induced CTLs could kill glioma U251 in vitro, which suggests that IL-13 Rα2 might have such an impact in vivo and thus recombinant IL-13Ra2 protein might be used as an anti-tumor vaccine, providing a promising new strategy for the treatment of brain malignant gliomas.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 263, Issue 2, 2010, Pages 172–175
نویسندگان
, , , , , , , , ,