کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168425 1092893 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
In vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage
چکیده انگلیسی

This study was designed to evaluate the in vitro effects of l-carnitine and glutamine (Gln) on the sperm quality parameters of liquid-stored rabbit semen maintained up to 24 h at 5 °C. Pooled and extended ejaculates were divided into two equal portions. l-Carnitine doses of 0.5, 1 and 2 mM were added to the first portion, and glutamine was added at the same doses to the second portion. All samples were cooled to 5 °C and examined at 0, 6, 12 and 24 h of liquid storage. Supplementation of the semen extender with three different doses of l-carnitine provided significant increases in the percentage of motile sperm at 12 h (P < 0.01), and 24 h (P < 0.001) and enabled significant protection of the sperm plasma membrane (P < 0.01) at 12 and 24 h of cool-storage, in comparison to the control samples. Only the 2 mM dose of l-carnitine significantly (P < 0.01) decreased the rate of acrosomal damage when compared to the control group. Furthermore, all doses of Gln caused a significant (P < 0.01) decrease in acrosomal damage at 6 h, and provided significant improvement (P < 0.01) in sperm motility, acrosomal and plasma membrane integrities at 12 and 24 h of liquid storage, when compared to the controls. In conclusion, the supplementation of liquid-stored rabbit semen with l-carnitine and Gln provided a protection for sperm against cool storage-induced functional and structural damages.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 68, Issue 3, June 2014, Pages 349–353
نویسندگان
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