Behaviour of water bound in bone marrow cells affected by organic solvents of different polarity

The behaviour of intracellular water affected by organic solvents of different polarity in partially dehydrated marrow cells obtained from tubular bones of broiler chickens was studied using 1H NMR spectroscopy at 210–290 K. The 1H NMR spectra of intracellular water include two signals which can be assigned to strongly (SAW, chemical shift of the proton resonance δH = 4–5 ppm) and weakly (WAW, δH = 1.2–1.7 ppm) associated waters which can be also divided into weakly (WBW, frozen at 250 < T < 273 K and changes in the Gibbs free energy ΔG > −0.8 kJ/mol) and strongly (SBW, unfrozen at T < 250 K, ΔG < −0.8 kJ/mol) bound intracellular waters. Solvents of different polarity such as dimethylsulfoxide-d6 (Me2SO-d6), acetonitrile-d3, and chloroform-d differently affect structure, Gibbs free energy, and molecular mobility of intracellular water. A maximal fraction of SBW in WAW and a minimal fraction of SBW in SAW are observed on absorption of acetonitrile (0.8 g/g) by cells. The opposite results are on addition of Me2SO (0.8 g/g) which strongly changes organisation of intracellular water and enhances the freezing point depression of SBW.