کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2171220 1093473 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of fibroblast growth factor on distinct differentiation potential of cord blood–derived unrestricted somatic stem cells and Wharton's jelly–derived mesenchymal stem/stromal cells
ترجمه فارسی عنوان
اثر فاکتور رشد فیبروبلاست در پتانسیل تمایز متمایزی از خون بند ناف سلول های بنیادی سنتی بدون محدودیت و سلول های ساقه و استروما مزانشیمی وارتون
کلمات کلیدی
عامل فیبروبلاست پایه، سلول های ساقه مغز / استرومال مزانشیمی، خون بند ناف، سلول های بنیادی بدون محدودیت، ژله وارتون
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی

Background aims. Perinatal tissues are considered an attractive source of mesenchymal stem/stromal cells (MSCs) and have unique characteristics depending on their origin. In this study, we compared the basic characteristics of unrestricted somatic stem cells isolated from cord blood (CB-USSCs) and MSCs isolated from Wharton's jelly of umbilical cords (WJ-MSCs). We also evaluated the effect of basic fibroblast growth factor (bFGF) supplementation on the growth and differentiation of these cells. Methods. CB-USSCs and WJ-MSCs were isolated from the same individual (n = 6), and their morphology, cell surface antigens, proliferation, expression of stemness markers and adipogenic, osteogenic and chondrogenic differentiation potentials were evaluated. Their morphology, proliferation and differentiation potentials were then also compared in the presence of bFGF supplementation (10 ng/mL). Results. Overall, CB-USSCs expressed DLK-1 and negative for all the HOX gene markers. The expression of cell surface antigen CD90, growth capacity and adipogenic differential potential of CB-USSCs were lower than those of WJ-MSCs. WJ-MSCs showed higher growth capacity, but the expression of CD73 and CD105 and their osteogenic differentiation potential were lower than those of CB-USSCs. The spindle morphology of both CB-USSCs and WJ-MSCs and the growth and adipogenic differentiation of CB-USSCs were improved by bFGF supplementation. However, the bFGF supplement did not have any positive effect on the tri-lineage differentiation potentials of WJ-MSCs. Conclusions. CB-USSCs and WJ-MSCs each had distinct characteristics including different growth capacity, distinguishable cell surface markers and distinct adipogenic and osteogenic potentials. bFGF supplementation improved the growth capacity and adipogenic differentiation of CB-USSCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 17, Issue 12, December 2015, Pages 1723–1731
نویسندگان
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