کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172543 1093552 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-yield expression, purification and characterization of tumor-targeted IFN-α2a
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
High-yield expression, purification and characterization of tumor-targeted IFN-α2a
چکیده انگلیسی

BackgroundIFN-α has been shown to be effective against hematologic malignancies. However, it is ineffective against most solid tumors and has not been satisfactory because of its toxicity.MethodsThe NGR (Asn-Gly-Arg) peptide is a tumor-homing peptide. In order to increase the anti-tumor activity of IFN-α2a and lower the dose, we coupled a cyclic NGR peptide with the C terminus of IFN-α2a (named IFN-α2a–NGR).ResultsThe fusion protein was expressed in E. coli and purified by ion-exchange chromatography. The purity of IFN-α2a–NGR was >98% and the final purification yield of IFN-α2a–NGR was approximately 18 mg/L. The anti-tumor efficacy and the binding ability of IFN-α2a–NGR with tumor vasculature were investigated in vitro and in vivo.DiscussionOur study has demonstrated that the anti-tumor efficacy of IFN-α2a–NGR is significantly increased in comparison with IFN-α2a, and IFN-α2a–NGR could selectively target tumor vessels. These data indicate that the tumor-homing peptide (NGR) can enhance the therapeutic efficacy of IFN-α2a against tumors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 9, Issue 1, 2007, Pages 60–68
نویسندگان
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