کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172798 1549564 2016 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional genomics identifies regulators of the phototransduction machinery in the Drosophila larval eye and adult ocelli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Functional genomics identifies regulators of the phototransduction machinery in the Drosophila larval eye and adult ocelli
چکیده انگلیسی


• Transcriptome profiling identified regulators of phototransduction machinery.
• Kr regulates Rh5 and Rh6 expression in the larval eye.
• Hazy, Lola, Camta and Dve regulate Rh2 expression in the adult ocelli.
• Kr, Hazy and Lola regulate phototransduction specific signaling molecules.

Sensory perception of light is mediated by specialized Photoreceptor neurons (PRs) in the eye. During development all PRs are genetically determined to express a specific Rhodopsin (Rh) gene and genes mediating a functional phototransduction pathway. While the genetic and molecular mechanisms of PR development is well described in the adult compound eye, it remains unclear how the expression of Rhodopsins and the phototransduction cascade is regulated in other visual organs in Drosophila, such as the larval eye and adult ocelli. Using transcriptome analysis of larval PR-subtypes and ocellar PRs we identify and study new regulators required during PR differentiation or necessary for the expression of specific signaling molecules of the functional phototransduction pathway. We found that the transcription factor Krüppel (Kr) is enriched in the larval eye and controls PR differentiation by promoting Rh5 and Rh6 expression. We also identified Camta, Lola, Dve and Hazy as key genes acting during ocellar PR differentiation. Further we show that these transcriptional regulators control gene expression of the phototransduction cascade in both larval eye and adult ocelli. Our results show that PR cell type-specific transcriptome profiling is a powerful tool to identify key transcriptional regulators involved during several aspects of PR development and differentiation. Our findings greatly contribute to the understanding of how combinatorial action of key transcriptional regulators control PR development and the regulation of a functional phototransduction pathway in both larval eye and adult ocelli.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 410, Issue 2, 15 February 2016, Pages 164–177
نویسندگان
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