کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2173112 1093696 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ENC1-like integrates the retinoic acid/FGF signaling pathways to modulate ciliogenesis of Kupffer’s vesicle during zebrafish embryonic development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
ENC1-like integrates the retinoic acid/FGF signaling pathways to modulate ciliogenesis of Kupffer’s vesicle during zebrafish embryonic development
چکیده انگلیسی

The left–right asymmetry is an essential feature shared by vertebrates. Cilia-driven counterclockwise flow in the mammalian node structure leads to the left–right asymmetric distribution of signals and subsequent asymmetric patterning. Although several signaling pathways have been identified in the specification of node ciliated cells, little is known about the direct downstream effectors of these signaling pathways. Here, we showed that zebrafish Ectoderm-Neural Cortex1-like (enc1l) is expressed in the Kupffer’s Vesicle (KV), an equivalent structure of the mammalian node in zebrafish, and is necessary for KV ciliogenesis. Loss-of-function of enc1l increased the number and decreased the length of KV cilia. The enc1l expression in the KV region was specifically regulated by retinoic acid (RA), FGF, and Wnt signaling pathways. In addition, knocking down enc1l or ectopic enc1l expression was able to rescue the KV cilium defects caused by alteration of RA and FGF signaling, but not Wnt signaling. Taken together, these data indicate thatEnc1l is a direct downstream effector of RA and FGF signaling pathways and modulates KV ciliogenesis in the zebrafish embryo.

Figure optionsDownload high-quality image (293 K)Download as PowerPoint slideHighlights
► Cilia-driven counterclockwise fluid flow in zebrafish Kupffer’s Vesicle (KV) resulted in left–right patterning during embryonic development.
► We find that Ectoderm-Neural Cortex 1-like (enc1l) is necessary for KV ciliogenesis in zebrafish embryo development.
► Loss-of-function of enc1l increased the number and decreased the length of KV cilia.
► The enc1l expression in the KV region was specifically regulated by retinoic acid (RA), FGF, and Wnt signaling pathways.
► Accordingly, knocking down Enc1l or ectopic enc1l expression was able to rescue the KV cilium defects caused by alteration of RA and FGF signaling, but not Wnt signaling.
► These data indicate that Enc1l is a direct downstream effector of RA and FGF signaling pathways and modulates KV ciliogenesis in the zebrafish embryo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 374, Issue 1, 1 February 2013, Pages 85–95
نویسندگان
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