کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2173377 | 1093717 | 2012 | 13 صفحه PDF | دانلود رایگان |

Epicardium-derived cells (EPDCs) invade the myocardium and differentiate into fibroblasts and vascular smooth muscle (SM) cells, which support the coronary vessels. The transcription factor Pod1 (Tcf21) is expressed in subpopulations of the epicardium and EPDCs in chicken and mouse embryonic hearts, and the transcription factors WT1, NFATC1, and Tbx18 are expressed in overlapping and distinct subsets of Pod1-expressing cells. Expression of Pod1 and WT1, but not Tbx18 or NFATC1, is activated with all-trans-retinoic acid (RA) treatment of isolated chick EPDCs in culture. In intact chicken hearts, RA inhibition leads to decreased Pod1 expression while RA treatment inhibits SM differentiation. The requirements for Pod1 in differentiation of EPDCs in the developing heart were examined in mice lacking Pod1. Loss of Pod1 in mice leads to epicardial blistering, increased SM differentiation on the surface of the heart, and a paucity of interstitial fibroblasts, with neonatal lethality. Epicardial epithelial-to-mesenchymal transition (EMT) and endothelial differentiation of coronary vessels are relatively unaffected. On the surface of the myocardium, expression of multiple SM markers is increased in Pod1-deficient EPDCs, demonstrating premature SM differentiation. Increased SM differentiation also is observed in Pod1-deficient lung mesenchyme. Together, these data demonstrate a critical role for Pod1 in controlling mesenchymal progenitor cell differentiation into SM and fibroblast lineages during cardiac development.
Figure optionsDownload high-quality image (174 K)Download as PowerPoint slideHighlights
► Pod1/Tcf21, WT1, NFATC1, and Tbx18 are expressed in subsets of EPDCs in the embryo.
► Retinoic acid selectively promotes Pod1 and WT1 expression in isolated chick EPDCs.
► Pod1 is required to promote EPDC differentiation into fibroblasts in mouse embryos. (85).
► Pod1−/− EPDCs prematurely differentiate into smooth muscle on the heart surface.
► Pod1−/− lung mesenchymal cells have increased smooth muscle gene expression.
Journal: Developmental Biology - Volume 368, Issue 2, 15 August 2012, Pages 345–357