کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2173455 1093723 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo calcium dynamics during neural crest cell migration and patterning using GCaMP3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
In vivo calcium dynamics during neural crest cell migration and patterning using GCaMP3
چکیده انگلیسی

Examining calcium dynamics within the neural crest (NC) has the potential to shed light on mechanisms that regulate complex cell migration and patterning events during embryogenesis. Unfortunately, typical calcium indicators are added to culture media or have low signal to noise after microinjection into tissue that severely limit analyses to cultured cells or superficial events. Here, we studied in vivo calcium dynamics during NC cell migration and patterning, using a genetically encoded calcium sensor, GCaMP3. We discovered that trunk NC cells displayed significantly more spontaneous calcium transients than cranial NC cells, and during cell aggregation versus cell migration events. Spontaneous calcium transients were more prevalent during NC cell aggregation into discrete sympathetic ganglia (SG). Blocking of N-cadherin activity in trunk NC cells near the presumptive SG led to a dramatic decrease in the frequency of spontaneous calcium transients. Detailed analysis and mathematical modeling of cell behaviors during SG formation showed NC cells aggregated into clusters after displaying a spontaneous calcium transient. This approach highlights the novel application of a genetically encoded calcium indicator to study subsets of cells during ventral events in embryogenesis.

Figure optionsDownload high-quality image (314 K)Download as PowerPoint slideHighlights
► First report of in vivo calcium transients during formation of sympathetic ganglia.
► Trunk neural crest in vivo more likely to exhibit calcium transients than cranial.
► Pattern of calcium transients correlates with formation of the sympathetic ganglia.
► Mathematical model shows calcium transients are correlated with cluster formation.
► Spontaneous calcium transients refine the pattern of sympathetic ganglia anlagen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 358, Issue 2, 15 October 2011, Pages 309–317
نویسندگان
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