کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2173517 1093726 2011 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pabp binds to the osk 3′UTR and specifically contributes to osk mRNA stability and oocyte accumulation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Pabp binds to the osk 3′UTR and specifically contributes to osk mRNA stability and oocyte accumulation
چکیده انگلیسی

RNA localization is tightly coordinated with RNA stability and translation control. Bicaudal-D (Bic-D), Egalitarian (Egl), microtubules and their motors are part of a Drosophila transport machinery that localizes mRNAs to specific cellular regions during oogenesis and embryogenesis. We identified the Poly(A)-binding protein (Pabp) as a protein that forms an RNA-dependent complex with Bic-D in embryos and ovaries. pabp also interacts genetically with Bic-D and, similar to Bic-D, pabp is essential in the germline for oocyte growth and accumulation of osk mRNA in the oocyte. In the absence of pabp, reduced stability of osk mRNA and possibly also defects in osk mRNA transport prevent normal oocyte localization of osk mRNA. pabp also interacts genetically with osk and lack of one copy of pabp+ causes osk to become haploinsufficient. Moreover, pointing to a poly(A)-independent role, Pabp binds to A-rich sequences (ARS) in the osk 3′UTR and these turned out to be required in vivo for osk function during early oogenesis. This effect of pabp on osk mRNA is specific for this RNA and other tested mRNAs localizing to the oocyte are less and more indirectly affected by the lack of pabp.


► Poly(A)-binding protein (Pabp) is in a complex with Bic-D.
► pabp interacts genetically with Bic-D and osk.
► pabp is essential for oocyte growth, and for stability and localization of osk mRNA.
► Pabp binds specifically to A-rich sequences (ARS) in the osk 3′UTR.
► osk 3′UTR ARS are required in vivo for osk function during early oogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 357, Issue 2, 15 September 2011, Pages 404–418
نویسندگان
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