کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2173743 1093747 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation
چکیده انگلیسی

Sperm motility is essential for achieving fertilization. In animals with external fertilization as amphibians, spermatozoa are stored in a quiescent state in the testis. Spermiation to hypotonic fertilization media triggers activation of sperm motility. Bufo arenarum sperm are immotile in artificial seminal plasma (ASP) but acquire in situ flagellar beating upon dilution. In addition to the effect of low osmolarity on sperm motility activation, we report that diffusible factors of the egg jelly coat (EW) regulate motility patterns, switching from in situ to progressive movement. The signal transduction pathway involved in amphibian sperm motility activation is mostly unknown. In the present study, we show a correlation between motility activation triggered by low osmotic pressure and activation of protein kinase A (PKA). Moreover, this is the first study to present strong evidences that point toward a role of a transmembrane adenyl-cyclase (tmAC) in the regulation of amphibian sperm motility through PKA activation.

Research Highlights
► Dilution into hypotonic media stimulates a tmAC in amphibian sperm.
► Both forskolin and cAMP trigger phosphorylation of PKA substrates and sperm motility.
► Transmembrane AC inhibitors diminished PKA activation and flagellar beating.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 350, Issue 1, 1 February 2011, Pages 80–88
نویسندگان
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