کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2173747 1093747 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interactions between oligodendrocyte precursors control the onset of CNS myelination
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Interactions between oligodendrocyte precursors control the onset of CNS myelination
چکیده انگلیسی

The formation of CNS myelin is dependent on the differentiation of oligodendrocyte precursor cells (OPCs) and oligodendrocyte maturation. How the initiation of myelination is regulated is unclear, but it is likely to depend on the development of competence by oligodendrocytes and receptivity by target axons. Here we identify an additional level of control of oligodendrocyte maturation mediated by interactions between the different cellular components of the oligodendrocyte lineage. During development oligodendrocyte precursors mature through a series of stages defined by labeling with monoclonal antibodies A2B5 and O4. Newly differentiated oligodendrocytes begin to express galactocerebroside recognized by O1 antibodies and subsequently mature to myelin basic protein (MBP)-positive cells prior to formation of compact myelin. Using an in vitro brain slice culture system that supports robust myelination, the consequences of ablating cells at different stages of the oligodendrocyte lineage on myelination have been assayed. Elimination of all OPC lineage cells through A2B5+, O4+, and O1+ complement-mediated cell lysis resulted in a delay in development of MBP cells and myelination. Selective elimination of early OPCs (A2B5+) also unexpectedly resulted in delayed MBP expression compared to controls suggesting that early OPCs contribute to the timing of myelination onset. By contrast, elimination of differentiated (O1+) immature oligodendrocytes permanently inhibited the appearance of MBP+ cells suggesting that oligodendrocytes are critical to facilitate the maturation of OPCs. These data illuminate that the presence of intra-lineage feed-forward and feedback cues are important for timely myelination by oligodendrocytes.

Research Highlights
► Oligodendrocytes follow a normal maturation process in a slice culture model.
► Specific stages of the OPC lineage can be ablated by complement-mediated cell lysis.
► Oligodendrocyte maturation and myelination delayed after ablation of OPCs.
► MBP+ cells fail to develop after ablation of O1 immature oligodendrocytes.
► PDGF partially overcomes O1+ cell ablation block of oligo maturation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 350, Issue 1, 1 February 2011, Pages 127–138
نویسندگان
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