کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2174917 1093825 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of Cajal–Retzius neurons in mouse forebrain is regulated by transforming growth factor β-Fox signaling pathways
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Generation of Cajal–Retzius neurons in mouse forebrain is regulated by transforming growth factor β-Fox signaling pathways
چکیده انگلیسی

The generation of Cajal–Retzius (CR) neurons is restricted to discrete sites in the telencephalon. Most of these sites do not express Foxg1, a transcription factor that inhibits transforming growth factor (TGF)β-dependent upregulation of p21. We tested the hypothesis that TGFβ signaling triggers CR neurogenesis in Foxg1-deficient zones through p21 induction. In Foxg1+/+ mice, p21 (a) was expressed in select cycling cells in CR neuron-producing areas and (b) was co-localized in newly generated CR neurons. Zones of CR neuronal production and p21 expression were expanded in the forebrains of Foxg1Cre/Cre mice. Manipulation of TGFβ signaling in explants from cortical hems of wild-type mice altered p21 expression and the production of CR neurons. Furthermore, despite continued TGFβ activity, p21 immunoreactivity diminished in CR neurons with distance from their generation site. This implicated a second pathway controlling p21 expression. We provide evidence that Foxo3a, which has been shown to translocate into the nucleus to act as a transcriptional co-activator of TGFβ-dependent upregulation of p21, is strategically expressed to be involved in controlling p21 expression in CR neurons. Specifically, Foxo3a was nuclear in p21+/reelin+ cells in sites of CR neuronal generation, however, nuclear Foxo3a immunoreactivity was absent in p21−/reelin+ cells distal from sites of CR neurogenesis. Thus, TGFβ and Foxo3a may work in concert to regulate expression of p21 during CR neuronal generation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 313, Issue 1, 1 January 2008, Pages 35–46
نویسندگان
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