کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2175218 | 1093834 | 2007 | 12 صفحه PDF | دانلود رایگان |
The establishment of axon–dendrite polarity in mammalian neurons has recently been shown to involve the kinases Akt and GSK-3β. Here we report the function of the integrin-linked kinase (ILK) in neuronal polarization. ILK distribution is differential: with more of it present in the axonal tips than that in the dendritic tips of a polarized neuron. Inactivation of ILK by chemical inhibitors, a kinase-inactive mutant or siRNAs inhibited axon formation, whereas a kinase hyperactive ILK mutant induced the formation of multiple axons. Biochemical studies indicate that ILK is upstream of Akt and GSK-3β. Manipulations of multiple intracellular components indicate that ILK is functionally upstream of Akt and GSK-3β but downstream of PI3K in neuronal polarity. These results reveal a key role of ILK in the formation of neuronal polarity and suggest a signaling pathway important for neuronal polarity.
Journal: Developmental Biology - Volume 306, Issue 2, 15 June 2007, Pages 457–468