کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2176120 1093863 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish
چکیده انگلیسی

We have investigated the role of Na,K-ATPase genes in zebrafish ear development. Six Na,K-ATPase genes are differentially expressed in the developing zebrafish inner ear. Antisense morpholino knockdown of Na,K-ATPase α1a.1 expression blocked formation of otoliths. This effect was phenocopied by treatment of embryos with ouabain, an inhibitor of Na,K-ATPase activity. The otolith defect produced by morpholinos was rescued by microinjection of zebrafish α1a.1 or rat α1 mRNA, while the ouabain-induced defect was rescued by expression of ouabain-resistant zebrafish α1a.1 or rat α1 mRNA. Knockdown of a second zebrafish α subunit, α1a.2, disrupted development of the semicircular canals. Knockdown of Na,K-ATPase β2b expression also caused an otolith defect, suggesting that the β2b subunit partners with the α1a.1 subunit to form a Na,K-ATPase required for otolith formation. These results reveal novel roles for Na,K-ATPase genes in vestibular system development and indicate that different isoforms play distinct functional roles in formation of inner ear structures. Our results highlight zebrafish gene knockdown-mRNA rescue as an approach that can be used to dissect the functional properties of zebrafish and mammalian Na,K-ATPase genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 294, Issue 1, 1 June 2006, Pages 148–160
نویسندگان
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