کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2176246 | 1093871 | 2006 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Calcineurin function is required for myofilament formation and troponin I isoform transition in Drosophila indirect flight muscle Calcineurin function is required for myofilament formation and troponin I isoform transition in Drosophila indirect flight muscle](/preview/png/2176246.png)
Mutations in the Drosophila calcineurin B2 gene cause the collapse of indirect flight muscles during mid stages of pupal development. Examination of cell fate-specific markers indicates that unlike mutations in genes such as vestigial, calcineurin B2 does not cause a shift in cell fate from indirect flight muscle to direct flight muscle. Genetic and molecular analyses indicate a severe reduction of myosin heavy chain gene expression in calcineurin B2 mutants, which accounts at least in part for the muscle collapse. Myofibrils in calcineurin B2 mutants display a variety of phenotypes, ranging from normal to a lack of sarcomeric structure. Calcineurin B2 also plays a role in the transition to an adult-specific isoform of troponin I during the late pupal stages, although the incompleteness of this transition in calcineurin B2 mutants does not contribute to the phenotype of muscle collapse. Together, these findings suggest a molecular basis for the indirect flight muscle hypercontractility phenotype observed in flies mutant for Drosophila calcineurin B2.
Journal: Developmental Biology - Volume 289, Issue 1, 1 January 2006, Pages 17–29