کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2176641 | 1549608 | 2014 | 16 صفحه PDF | دانلود رایگان |
• PDGF-treated fibroblasts display rapid, persistent migration on a line substrate
• A front module of podosome-like structures coordinates key aspects of cell polarity
• A back myosin-based module solely regulates tail retraction
• Front/back uncoupling is necessary for robust persistent migration
SummaryDirectional migration requires robust front/back polarity. We find that fibroblasts treated with platelet-derived growth factor (PDGF) and prepolarized by plating on a fibronectin line substrate exhibit persistent migration for hours. This does not occur in the absence of PDGF or on uniformly coated fibronectin substrates. Persistent migration arises from establishment of two functional modules at cell front and back. At the front, formation of a zone containing podosome-like structures (PLS) dynamically correlates with low RhoA and myosin activity and absence of a contractile lamella. At the back, myosin contractility specifically controls tail retraction with minimal crosstalk to the front module. The PLS zone is maintained in a dynamic steady state that preserves size and position relative to the cell front, allowing for long-term coordination of front and back modules. We propose that front/back uncoupling achieved by the PLS zone is crucial for persistent migration in the absence of directional cues.
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Journal: - Volume 30, Issue 6, 29 September 2014, Pages 701–716