کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2176660 1094561 2014 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional Genomic Analysis of the Periodic Transcriptome in the Developing Drosophila Wing
ترجمه فارسی عنوان
تجزیه و تحلیل ژنومی کاربردی ترانزیستوروم دوره ای در حال توسعه درزوفیلا بال
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• The cell-cycle-associated transcriptome of Drosophila is highly context dependent
• RNAi screen validates more than 100 periodic genes implicated in wing growth
• Genes controlling cell-cycle phasing, mitosis, and other processes are reported
• A putative long ncRNA, CR32027, is required for IKNM but not mitotic progression

SummaryThe eukaryotic cell cycle, driven by both transcriptional and posttranslational mechanisms, is the central molecular oscillator underlying tissue growth throughout animals. Although genome-wide studies have investigated cell-cycle-associated transcription in unicellular systems, global patterns of periodic transcription in multicellular tissues remain largely unexplored. Here we define the cell-cycle-associated transcriptome of the developing Drosophila wing epithelium and compare it with that of cultured Drosophila S2 cells, revealing a core set of periodic genes and a surprising degree of context specificity in periodic transcription. We further employ RNAi-mediated phenotypic profiling to define functional requirements for more than 300 periodic genes, with a focus on those required for cell proliferation in vivo. Finally, we investigate uncharacterized genes required for interkinetic nuclear migration. Combined, these findings provide a global perspective on cell-cycle control in vivo, and they highlight a critical need to understand the context-specific regulation of cell proliferation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 29, Issue 1, 14 April 2014, Pages 112–127
نویسندگان
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