TRIM Proteins Regulate Autophagy and Can Target Autophagic Substrates by Direct Recognition

• The TRIM family of proteins regulates autophagy and directs selective autophagy
• TRIMs serve as platforms for the assembly of the autophagic initiation machinery
• TRIM5α is an autophagic receptor governing selective autophagy of HIV-1 capsid

SummaryAutophagy, a homeostatic process whereby eukaryotic cells target cytoplasmic cargo for degradation, plays a broad role in health and disease states. Here we screened the TRIM family for roles in autophagy and found that half of TRIMs modulated autophagy. In mechanistic studies, we show that TRIMs associate with autophagy factors and act as platforms assembling ULK1 and Beclin 1 in their activated states. Furthermore, TRIM5α acts as a selective autophagy receptor. Based on direct sequence-specific recognition, TRIM5α delivered its cognate cytosolic target, a viral capsid protein, for autophagic degradation. Thus, our study establishes that TRIMs can function both as regulators of autophagy and as autophagic cargo receptors, and reveals a basis for selective autophagy in mammalian cells.

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