Multiclassifier Proteomics to Define Complexes Yields New Chromosomal Proteins

In a recent issue of Cell, Ohta et al. report a method of quantitative proteomics coupled with bioinformatic analysis for the identification of associated components in complex mixtures. Using this approach, they assayed the protein composition of mitotic chromosomes, identifying 4029 associated proteins, 562 of which are previously uncharacterized.