کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2177403 | 1094645 | 2008 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An Elmo-like Protein Associated with Myosin II Restricts Spurious F-Actin Events to Coordinate Phagocytosis and Chemotaxis An Elmo-like Protein Associated with Myosin II Restricts Spurious F-Actin Events to Coordinate Phagocytosis and Chemotaxis](/preview/png/2177403.png)
SummaryElmo proteins positively regulate actin polymerization during cell migration and phagocytosis through activation of the small G protein Rac. We identified an Elmo-like protein, ElmoA, in Dictyostelium discoideum that unexpectedly functions as a negative regulator of actin polymerization. Cells lacking ElmoA display an elevated rate of phagocytosis, increased pseudopod formation, and excessive F-actin localization within pseudopods. ElmoA associates with cortical actin and myosin II. TIRF microscopic observations of functional ElmoA-GFP reveal that a fraction of ElmoA localizes near the presumptive actin/myosin II cortex and the levels of ElmoA and myosin II negatively correlate with that of polymerizing F-actin. F-actin-regulated dynamic dispersions of ElmoA and myosin II are interdependent. Taken together, our data suggest that ElmoA modulates actin/myosin II at the cortex to prevent excessive F-actin polymerization around the cell periphery, thereby maintaining proper cell shape during phagocytosis and chemotaxis.
Journal: - Volume 15, Issue 4, 14 October 2008, Pages 590–602