کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2177996 1549619 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phenotypic and molecular genetic analysis of Pyruvate Kinase deficiency in a Tunisian family
ترجمه فارسی عنوان
تجزیه و تحلیل ژنتیکی فنوتیپ و مولکولی کمبود پیروات کیناز در خانواده تونس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی

Pyruvate Kinase (PK) deficiency is the most frequent red cell enzymatic defect responsible for hereditary non-spherocytic hemolytic anemia. The disease has been studied in several ethnic groups. However, it is yet an unknown pathology in Tunisia. We report here, the phenotypic and molecular investigation of PK deficiency in a Tunisian family.This study was carried out on two Tunisian brothers and members of their family. Hematological, biochemical analysis and erythrocyte PK activity were performed. The molecular characterization was carried out by gene sequencing technique.The first patient died few hours after birth by hydrops fetalis, the second one presented with neonatal jaundice and severe anemia necessitating urgent blood transfusion. This severe clinical picture is the result of a homozygous mutation of PKLR gene at exon 8 (c.1079G>A; p.Cys360Tyr).Certainly, this research allowed us to correlate the clinical phenotype severity with the identified mutation. Moreover, this will help in understanding the etiology of unknown anemia in our country.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Egyptian Journal of Medical Human Genetics - Volume 17, Issue 3, July 2016, Pages 265–270
نویسندگان
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