کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2178360 1549678 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Experimental approaches to investigate effector translocation into host cells in the Ustilago maydis/maize pathosystem
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Experimental approaches to investigate effector translocation into host cells in the Ustilago maydis/maize pathosystem
چکیده انگلیسی

The fungus Ustilago maydis is a pathogen that establishes a biotrophic interaction with Zea mays. The interaction with the plant host is largely governed by more than 300 novel, secreted protein effectors, of which only four have been functionally characterized. Prerequisite to examine effector function is to know where effectors reside after secretion. Effectors can remain in the extracellular space, i.e. the plant apoplast (apoplastic effectors), or can cross the plant plasma membrane and exert their function inside the host cell (cytoplasmic effectors). The U. maydis effectors lack conserved motifs in their primary sequences that could allow a classification of the effectome into apoplastic/cytoplasmic effectors. This represents a significant obstacle in functional effector characterization. Here we describe our attempts to establish a system for effector classification into apoplastic and cytoplasmic members, using U. maydis for effector delivery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 94, Issues 7–9, July–September 2015, Pages 349–358
نویسندگان
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