Targeting of Pex8p to the peroxisomal importomer

Pex8p of Saccharomyces cerevisiae is located at the inner surface of the peroxisomal membrane and is essential for the assembly of the importomer, a multi-protein complex of the peroxisomal protein import machinery. By means of the yeast two-hybrid system as well as in vitro binding studies, we demonstrate that Pex8p is capable to interact with the PTS2-receptor Pex7p and the docking complex component Pex13p with its C-terminal SH3-domain providing the binding site. Analysis of the importomer composition of pex-mutants revealed that both PTS-receptors as well as the auxiliary proteins Pex18p and Pex21p are neither required for Pex8p association with the importomer nor for its function as an organizer of the peroxisomal protein import machinery.