کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2178761 1549715 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane palmitoylated proteins regulate trafficking and processing of nectins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Membrane palmitoylated proteins regulate trafficking and processing of nectins
چکیده انگلیسی

Nectins are cell–cell adhesion molecules involved in the formation of various intercellular junctions and the establishment of apical-basal polarity at cell–cell adhesion sites. To have a better understanding of the roles of nectins in the formation of cell–cell junctions, we searched for new cytoplasmic binding partners for nectin. We report that nectin-1α associates with membrane palmitoylated protein 3 (MPP3), one of the human homologues of a Drosophila tumor suppressor gene, Disc large. Two major forms of MPP3 at 66 and 98 kDa were detected, in conjunction with nectin-1α, suggesting that an association between the two may occur in various cell types. Nectin-1α recruits MPP3 to cell–cell contact sites, mediated by a PDZ-binding motif at the carboxyl terminus of nectin-1α. Association with MPP3 increases cell surface expression of nectin-1α and enhances nectin-1α ectodomain shedding, indicating that MPP3 regulates trafficking and processing of nectin-1α. Further study showed that MPP3 interacts with nectin-3α, but not with nectin-2α, showing that the association of nectins with MPP3 is isoform-specific. MPP5, another MPP family member, interacts with nectins with varying affinity and facilitates surface expression of nectin-1α, nectin-2α, and nectin-3α. These data suggest that wide interactions between nectins and MPP family members may occur in various cell–cell junctions and that these associations may regulate trafficking and processing of nectins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 90, Issue 5, May 2011, Pages 365–375
نویسندگان
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