کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2179198 1549772 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein kinase A targeting and activation as seen by small-angle solution scattering
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Protein kinase A targeting and activation as seen by small-angle solution scattering
چکیده انگلیسی

We have studied the solution structures of the multi-functional protein kinase A using small-angle X-ray and neutron scattering and have found a remarkable structural diversity in the different isoforms of this multi-subunit enzyme, in spite of its having high sequence homology and a common domain organization within its sequences. The available high-resolution crystal and NMR structural data for the protein kinase A components have aided in the interpretation of the solution scattering data and enabled us to develop models that bring insights into protein kinase A activation and targeting mechanisms, such as the opening and closing of the catalytic cleft to facilitate substrate binding or inhibition, respectively, and the role of sequence segments that join functional domains in the R subunit in providing a structurally flexible scaffold for interactions with the C subunit and A kinase-anchoring proteins (AKAPs).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 85, Issue 7, 5 July 2006, Pages 655–662
نویسندگان
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